OUR PRODUCTS
smart Blood DNA Midi prep (m)

Product Details
  • Easiest handling thanks to SmartExtraction-Technology
  • Low need of resources: DNA isolation using a thermal shaker (e.g. Biometra TS1 ThermoShaker or Biometra TSC ThermoShaker)
  • Minimal shearing power for purification of high molecular weight DNA up to 500 kbp
  • Unique Smart Modified Surfaces for optimal binding capacity and high yields
  • Innovative 3-step-lysis consisting of lysis of erythrocytes, collection and digestion of DNA carrying cells

The smart Blood DNA Midi prep (m) is based on manual application of SmartExtraction technology, which allows binding of genomic DNA to the unique Smart Modified Surfaces. Up to 10 ml whole can be prepared. The required lab equipment is minimal and just limited to a thermal shaker, like the Biometra TS1 ThermoShaker or Biometra TSC ThermoShaker and a magnet rack. In addition to the 3-step-lysis consisting of lysis of erythrocytes, collection and digest of DNA carrying cells, DNA is efficiently bound, washed and eluted. The routine reduces the shearing power to the nucleic acid to an absolute minimum. The isolated DNA has a high molecular weight up to 500 kbp and shows best quality. Direct downstream applications are immediately possible.

Starting material

  • Whole blood samples of 0.5 to 10 ml
  • Fresh or frozen blood
  • Stabilizers: EDTA, citrate or heparin

Average Yield

Depends on sample and used volume

  • 0.5 ml whole blood: approx. 5 – 15 μg gDNA
  • 1.0 ml whole blood: approx. 15 – 30 μg gDNA
  • 2.0 ml whole blood: approx. 40 – 70 μg gDNA
  • 3.0 ml whole blood: approx. 50 – 90 μg gDNA
  • 5.0 ml whole blood: approx. 90 – 160 μg gDNA
  • 10.0 ml whole blood: approx. 160 – 320 μg gDNA

Extraction Time

  • Lysis: depending on starting material
  • Extraction: approx. 35 min

Volume

  • 200 – 1000 μl Elution Buffer

Quality

  • 1.7 – 2.0

เอกสารเพิ่มเติม

1. Brochure_SmartExtraction_EN

2. MSDS_smart_Blood__DNA_Midi_prep_m

3. Manual_smart_Blood_DNA_Midi_prep_m

Size
Order number Amount
845-KS-8100010 10 reactions
845-KS-8100050 50 reactions
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บริษัท แลบคอนเนคชั่น จำกัด (สำนักงานใหญ่) เลขที่ 335 ม.14
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บริษัท แลบคอนเนคชั่น จำกัด (สาขา 1) เลขที่ 156/101 ม.10 อาคารสุเทพวิวแมนชั่น ชั้น1 ถ.คันคลองชลประทาน
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