The innuPREP Mycobacteria DNA Kit selectively extracts mycobacterial DNA from sputum, bronchoalveolar lavages or tissue samples (e. g. lymph nodes). The extraction process includes a step in which samples are pretreated with N-Acetyl cysteine followed by digestion with lysozyme and Proteinase K. After sample digestion, DNA is bound (under optimized binding conditions) to a Spin Filter membrane, washed and then desorbed from the filter membrane through the addition of a low-salt buffer. Special protocols adapted to different starting materials ensure maximum DNA yields and outstanding purity.
Sputum samples, 0.2 – 5.0 ml
Bronchoalveolar lavages, up to 1.0 ml
Tissues (e. g., lymph nodes) ranging in size from 1.0 mg to no more than 10 mg
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