OUR PRODUCTS
innuPREP Plasmid Mini Kit 2.0

Product Details
  • Allows preparation of a wide volume range between 0.5 ml to 15 ml bacterial culture
  • Purification of up to 80 µg high qualitative plasmid DNA
  • Optimized Spin Filter membranes with adapted binding capacity
  • Accelerated routines thanks to optimized protocols
  • Direct use of high qualitative plasmid DNA for sensitive applications, like transfection, cloning, PCR or sequencing
  • Comfortable handling due to mini Spin Filter format (after lysis)

innuPREP Plasmid Mini Kit 2.0 allows the fast and easy isolation of plasmid DNA from bacteria cultures. To gain an optimal result protocol sequences, as well as the chemistry and used Spin Filters were adapted if compared to precursor products innuPREP Plasmid Mini Kit and innuPREP Plasmid Mini Kit Plus. The routine is based on the combination of an alkaline lysis and binding of the plasmid DNA to a filter membrane after precipitation of the chromosomal DNA and bacterial proteins. Bound plasmid DNA is then washed and finally eluted by using a low molar buffer. The isolated plasmid DNA can be used for a number of sensitive downstream applications, like transfection, cloning, PCR or sequencing directly. The new procedure also allows for processing of 0.5 ml to 15 ml of bacterial cultures based on the usage of a table centrifuge (after lysis). Due to the new filter membrane up to 80 µg pDNA can be isolated from one sample.

Starting material

  • Bacteria cultures (0.5 to 15 ml)
  • Extraction of high copy plasmid DNA/ low copy plasmid DNA, P1 constructs and cosmid DNA

Average yield

  • Depending on the sample and the used volume
  • Typical yield from 2 ml (high copy Plasmid): approx. 6 – 20 µg
  • Typical yield from 15 ml (high copy Plasmid): approx. 60 – 70 µg

Extraction time

  • Approx. 15 min

Binding capacity

  • Approx. 80 µg pDNA

Average purity

  • 1,7 – 2,0

 

เอกสารเพิ่มเติม

1. Manual_innuPREP_Plasmid_Mini_Kit_2.0

2. SDS_innuPREP_Plasmid_Mini_Kit_2_0_en

3. Short_Manual_innuPREP_Plasmid_Mini_Kit_Protocol_1

4. Short_Manual_innuPREP_Plasmid_Mini_Kit_Protocol_2

Size
Order number
(Spin Filter
without lid)
Order number
(Spin Filter
with lid)
Amount
845-KS-5040010 845-KS-5041010 10 reactions
845-KS-5040050 845-KS-5041050 50 reactions
845-KS-5040250 845-KS-5041250 250 reactions
845-KS-5040500 845-KS-5041500 500 reactions
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