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innuPREP Micro RNA Kit

analytik-jena logoProduct Details

  • Optimized Binding Buffer system for recovering large amounts of small RNA molecules and total RNA
  • Selective removal of genomic DNA prevents DNase I digestion
  • Easy to use; no toxic β-mercaptoethanol

Using the innuPREP Micro RNA Kit allows researchers to isolate small RNA molecules and achieve high yields. The new, optimized Binding Buffer system makes it possible to achieve high rates of recovery for small RNA molecules such as mRNA, tRNA, rRNA and snRNA. The first step utilizes well-established Spin Filter column technology to selectively remove genomic DNA; the RNA is then bound, washed and finally removed from the filter membrane using RNase-free water. Users have the flexibility to adjust the elution volume within a range of 30 µl to 80 µl. Also, the extraction chemistry (DC-Technologie) renders the use of highly toxic β-mercaptoethanol utterly unnecessary for isolating RNA.
The innuPREP Bacteria Lysis Booster is also available to further optimize the lysis of bacterial cells, particularly with regard to bacterial strains that are difficult to lyse.

Starting material

  • Eucaryotic cells (max. 5 × 106)
  • Tissue samples (max. 20 mg)
  • Gram+ and gram– bacteria (max. 1 × 109)
  • Biopsies

Extraction time

  • Approx. 15 – 40 minutes

Average yield

  • Depends on the type and quantity of the starting material
  • Up to 100 µg RNA
  • High recovery rate for small RNA molecules

Binding capacity

  • Column binding capacity: approx. 100 µg RNA

Average purity

  • 1,8 – 2,1

เอกสารเพิ่มเติม

1. Manual_innuPREP_Micro_RNA_Kit

2. SDS_innuPREP_Micro_RNA_Kit_en

3. innuPREP_Micro_RNA_Kit_-_short_protocol_1

4. innuPREP_Micro_RNA_Kit_-_short_protocol_2

5. innuPREP_Micro_RNA_Kit_-_short_protocol_3

Size
Order number Quantity
845-KS-2030010 10 reactions
845-KS-2030050 50 reactions
845-KS-2030250 250 reactions
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