Optimized Binding Buffer system for recovering large amounts of small RNA molecules and total RNA
Selective removal of genomic DNA prevents DNase I digestion
Easy to use; no toxic β-mercaptoethanol
Using the innuPREP Micro RNA Kit allows researchers to isolate small RNA molecules and achieve high yields. The new, optimized Binding Buffer system makes it possible to achieve high rates of recovery for small RNA molecules such as mRNA, tRNA, rRNA and snRNA. The first step utilizes well-established Spin Filter column technology to selectively remove genomic DNA; the RNA is then bound, washed and finally removed from the filter membrane using RNase-free water. Users have the flexibility to adjust the elution volume within a range of 30 µl to 80 µl. Also, the extraction chemistry (DC-Technologie) renders the use of highly toxic β-mercaptoethanol utterly unnecessary for isolating RNA. The innuPREP Bacteria Lysis Booster is also available to further optimize the lysis of bacterial cells, particularly with regard to bacterial strains that are difficult to lyse.
Eucaryotic cells (max. 5 × 106)
Tissue samples (max. 20 mg)
Gram+ and gram– bacteria (max. 1 × 109)
Approx. 15 – 40 minutes
Depends on the type and quantity of the starting material
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