Includes CarrierMix with internal DNA and RNA extraction control
The innuPREP Virus DNA/RNA Kit- KFml is an extraction system for isolating viral DNA and RNA simultaneously. The kit is suitable for use with an extremely wide variety of starting materials. Binding nucleic acids to magnetic particles forms the basis for the automated routine, along with the use of the KingFisher ml processor. Up to 15 samples can be processed in parallel following a fast, highly efficient lysis step. Excellent yields of extremely high-quality nucleic acids are eluted in the final step and the resulting DNA can be used directly in downstream applications.
Cell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl)
Cell culture supernatant, enrichment medium (up to 200 µl)
Tissue samples (approx. 1- 5 mg)
Lysis: approx. 15-30 minutes, up to 60 minutes for tissue samples
KingFisher ml protocol: approx. 30 minutes
Quality of viral nucleic acids
Tested with positive results in cDNA synthesis, PCR and TaqMan real-time PCR
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