Contains all of the necessary consumables and PCR components
Sensitive, highly reproducible detection of all members of the Ixodidae family
The innuAMP Tick DNA Test provides verification of tick-specific DNA and, as such, can be used to determine how well a nucleic acid isolated from ticks can be amplified - an especially important feature with respect to subsequent diagnostic tests for detecting bacterial pathogens. In other words, the use of the innuAMP Tick DNA Test prevents false negatives in downstream nucleic acid studies. The amplification protocols for the tick-specific mitochondrial gene have been adapted to unique rapidPCR technology using either a Low-Profile Rapid (LPR) block or a Standard Profile Rapid (SPR) block (Analytik Jena AG). The protocols have also been optimized for use with PCR thermal cyclers such as the FlexCycler (Analytik Jena). The kit also contains all of the necessary consumables, PCR components and a positive control.
The test has been developed to detect DNA from all members of the Ixodidae family (hard-bodied ticks).
rapidPCR (SpeedCycler²): approx. 30 minutes
Standard PCR: Depends on thermal cyclers, approx. 2 hours
Approx. 20 minutes at 100 mA
Visible 150 bp fragment on a 2 % agarose gel after electrophoresis
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